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It is possible to display UV traces by selecting them on the MS Browser and then clicking on the ‘Open new Chromatogram’ icon . From here you will also be able to select a range in the wavelength:

Here you can see the result of having extracted the trace at 254nm:

And here you can see the result after having extracted the Total Absorbance Chromatogram:

From the PDA-Total Absorbance Chromatogram, you will be able to extract the complete UV spectrum.

To select a UV spectra: the dataset must have the full UV spectra set (and not just a single or a set of UV chromatograms, ie, MS browser panel must show a sub-item called DAD under the Traces item).

Using the crosshair mode and clicking on a UV chromatogram while holding Alt pressed, will display the UV spectrum for the clicked retention time.

You can also Co-add UV chromatograms by clicking and dragging the crosshair to select a range on DAD chromatograms as you can do with the TICs.

Download the files used at this tutorial.

Last modified: October 8, 2013 by Olalla Lema