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Analysis for the determination of a biologic drug’s higher order structure

Designed for the NMR analysis of biotherapeutics, like monoclonal antibodies. 2D NMR spectra of reference samples are compared with that of each test sample using a fingerprinting, or spectral similarity determination.

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New features

What’s new in Mnova BioHOS 3.0?

  • MCR Tool for Peak Purity Assessment in LCMS Data. (new with Mnova 15.1)
  • PLS Regression: Analyze and predict dependent variables with graphical tools.
  • SIMCA Classification: Supervised data classification method.
  • Enhanced PCA Analysis: Quantile Plots (1D NMR), comparison tool, plot labels, improved interactivity, and more distance options.
  • PROFILE Analysis: Export fingerprint spectra and sync plot crosshairs.
  • CCSD Peaks: Auto-detect reference peaks.
  • Data Preparation: Probabilistic Quotient Normalization (PQN) with result visualization.
Whats-new-Mnova-BioHOS
BioHOS

Benefits

HOS evaluation of medium to high molecular weight species.

Quality Assessment of Biologic drugs using 2D NMR spectral data.

Includes all the «Advanced Chemometrics» capabilities (PCA, PLS, and SIMCA) and 1D Profile, CCSD, and ECHOS.

Signal processing such as compressed sensing, denoising, data alignment, and 2D cross-peak analysis.

Matrix evaluations of all spectra to compare intra- and inter-class samples.

Full automation capabilities that allow the saving and reuse of analysis methods.

Mnova BioHOS – NIST mAb data

Reference: Robert G. Brinson at al. (2019) Enabling adoption of 2D-NMR for the higher order structure assessment of monoclonal antibody therapeutics, mAbs, 11:1, 94-105
DOI: 10.1080/19420862.2018.1544454

Data from the NISTaMb study and reference material are available here

BioHOS

Features

New MCR Tool for Peak Purity Assessment in LCMS Data

With the release of Mnova 15.1, we introduce an MCR tool based on the MCR-ALS algorithm for peak purity assessment, providing comprehensive tables and plots to streamline LCMS data analysis and enhance decision-making. This tool is available with the Advanced Chemometrics or BioHOS plugins.

Note: This tool requires an MSChrom plugin license.

MCR

New in Mnova 15.1

ECHOS Analysis

These are some of the analysis you can carry out with Mnova BioHOS «ECHOS Analysis»:

  • Pairwise intensity comparisons of points with the same coordinates in the reference – and test spectra.
  • A regression line is computed for the scatter plot of points. Manual and automatic noise cancellation.
  • Adjust threshold for residuals.

CCSD Comparison

With Mnova BioHOS «CCSD Analysis», you can:

  • Match selected peaks in reference and test spectra to calculate CCSD (average and per peak pair).
  • Rank test spectra by similarity to the reference.
  • Plot CCSD values and amplitude ratios per peak pair for quick inspection.

PCA Analysis

With Mnova BioHOS «PCA analysis», you can perform:

  • Data management: adjust parameters, binning, checks, filtering, normalization, and scaling.
  • Interactive PCA plots linked to spectra.
  • Plot templates: Explained Variance, Influence, Scores, Loadings.
  • Model building and validation.
  • Test new spectra against Reference PCA.

1D Profile

This feature leverages 1D NMR spectra to detect and evaluate small spectral changes, performing detailed statistical analysis.

  • Typically uses 1D 1H spectra.
  • Quantifies small differences.
  • Statistically treats multiple spectra.
BioHOS

Videos

Help & Resources

Guides & Manuals

BioHOS - User Manual

Resources

Evaluation of the Higher Order Structure of Biologics at Atomic Resolution with NMR
BioHOS

Related Applications

Academic, Government & Industrial

Target Audience

Organic Chemists
Researchers
Medicinal Chemists
BIOHOS

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